• Mycoplasma Growth Supplement, 10x solution

Mycoplasma Growth Supplement, 10x solution

AUD 775.00    Excl.GST
  • Product Code: GMNB-MGS1001
  • Availability: Pre-Order

Tags: Mycoplasma Growth Supplement

Mycoplasma Growth Supplement, 10x solution, 250ml

0.22 µm sterile filtration


Mycoplasma Growth Supplement is used in broth and agar media to culture mycoplasma species. The 10x solution is an ingredient in ATCC Medium 988 (Spiroplasma SP-4 Medium). It can also work with PPLO Broth for general Mycoplasma culturing.


Use

  • Enrichment and isolation of Mycoplasma spp. in broth and agar media
  • Formulation ingredients for ATCC Medium 988 (Spiroplasma SP-4)
  • Compatible with PPLO Broth for general mycoplasma culture


Cautions

  • Perform all manipulations in a biosafety cabinet or equivalent containment
  • Observe biosafety guidelines when handling pathogenic organisms


Pack

  • 250 mL sterile solution


Per 1000mL of 10X solution contains

Amino Acids 

 

L-Alanine

0.025 g

L-Arginine

0.0579 g

L-Aspartic acid

0.03 g

L-Cysteine-HCl

0.26 g

L-Cystine

0.02 g

L-Glutamic acid

0.075 g

L-Glutamine

0.1 g

Glycine

0.05 g

L-Histidine

0.02 g

Trans-4-Hydroxy-L-Proline

0.01 g

L-Isoleucine

0.02 g

L-Leucine

0.06 g

L-Lysine

0.07 g

L-Methionine

0.015 g

L-Phenylalanine

0.025 g

L-Proline

0.04 g

L-Serine

0.025 g

L-Threonine

0.03 g

L-Tryptophan

0.01 g

L-Tyrosine

0.04 g

L-Valine

0.025 g


Vitamins and Cofactors

 

L-Ascorbic acid

50 mg

p-Aminobenzoic acid (PABA)

0.05 mg

D-Biotin

0.01 mg

Choline chloride

0.5 mg

Coenzyme A · Na

2.5 mg

Cocarboxylase (thiamine pyrophosphate)

0.1 mg

Flavin adenine dinucleotide · 2Na

0.106 mg

Folic acid

0.01 mg

Niacinamide

0.025 mg

Nicotinic acid

0.025 mg

D-Pantothenic acid (hemicalcium)

0.01 mg

Pyridoxal-HCl 

0.025 mg

Pyridoxine-HCl

0.025 mg

Riboflavin

0.01 mg

Thiamine-HCl

0.01 mg


Nucleosides

 

2′-Deoxyadenosine

0.01 g

2′-Deoxyguanosine

0.01 g

2′-Deoxycytidine-HCl

0.0116 g

Thymidine

0.01 g

Uridine-5′-Triphosphate · Na

0.001 g

5-Methyldeoxycytidine

0.0001 g


Salts and Carbon Sources

 

CaCl2

0.2 g

MgSO4

0.0977 g

KCl

0.4 g

CH3COONa

0.05 g

NaCl

6.8 g

NaH2PO4

0.122 g

Glucose

1.0 g


Other Components

 

Glutathione

10 mg

D-Glucuronic acid · Na

3.88 mg

Cholesterol 

0.2 mg

Tween 80

5 mg

Phenol red · Na

21.24 mg


Storage

  • Aliquot unused portions into sterile tubes
  • Store at 4 °C. Use within recommended shelf-life to maintain sterility and activity