Mycoplasma Growth Supplement, 10x solution
- Product Code: GMNB-MGS1001
- Availability: Pre-Order
Mycoplasma Growth Supplement, 10x solution, 250ml
0.22 µm sterile filtration
Mycoplasma Growth Supplement is used in broth and agar media to culture mycoplasma species. The 10x solution is an ingredient in ATCC Medium 988 (Spiroplasma SP-4 Medium). It can also work with PPLO Broth for general Mycoplasma culturing.
Use
- Enrichment and isolation of Mycoplasma spp. in broth and agar media
- Formulation ingredients for ATCC Medium 988 (Spiroplasma SP-4)
- Compatible with PPLO Broth for general mycoplasma culture
Cautions
- Perform all manipulations in a biosafety cabinet or equivalent containment
- Observe biosafety guidelines when handling pathogenic organisms
Pack
- 250 mL sterile solution
Per 1000mL of 10X solution contains
Amino Acids |
|
L-Alanine |
0.025 g |
L-Arginine |
0.0579 g |
L-Aspartic acid |
0.03 g |
L-Cysteine-HCl |
0.26 g |
L-Cystine |
0.02 g |
L-Glutamic acid |
0.075 g |
L-Glutamine |
0.1 g |
Glycine |
0.05 g |
L-Histidine |
0.02 g |
Trans-4-Hydroxy-L-Proline |
0.01 g |
L-Isoleucine |
0.02 g |
L-Leucine |
0.06 g |
L-Lysine |
0.07 g |
L-Methionine |
0.015 g |
L-Phenylalanine |
0.025 g |
L-Proline |
0.04 g |
L-Serine |
0.025 g |
L-Threonine |
0.03 g |
L-Tryptophan |
0.01 g |
L-Tyrosine |
0.04 g |
L-Valine |
0.025 g |
Vitamins and Cofactors |
|
L-Ascorbic acid |
50 mg |
p-Aminobenzoic acid (PABA) |
0.05 mg |
D-Biotin |
0.01 mg |
Choline chloride |
0.5 mg |
Coenzyme A · Na |
2.5 mg |
Cocarboxylase (thiamine pyrophosphate) |
0.1 mg |
Flavin adenine dinucleotide · 2Na |
0.106 mg |
Folic acid |
0.01 mg |
Niacinamide |
0.025 mg |
Nicotinic acid |
0.025 mg |
D-Pantothenic acid (hemicalcium) |
0.01 mg |
Pyridoxal-HCl |
0.025 mg |
Pyridoxine-HCl |
0.025 mg |
Riboflavin |
0.01 mg |
Thiamine-HCl |
0.01 mg |
Nucleosides |
|
2′-Deoxyadenosine |
0.01 g |
2′-Deoxyguanosine |
0.01 g |
2′-Deoxycytidine-HCl |
0.0116 g |
Thymidine |
0.01 g |
Uridine-5′-Triphosphate · Na |
0.001 g |
5-Methyldeoxycytidine |
0.0001 g |
Salts and Carbon Sources |
|
CaCl2 |
0.2 g |
MgSO4 |
0.0977 g |
KCl |
0.4 g |
CH3COONa |
0.05 g |
NaCl |
6.8 g |
NaH2PO4 |
0.122 g |
Glucose |
1.0 g |
Other Components |
|
Glutathione |
10 mg |
D-Glucuronic acid · Na |
3.88 mg |
Cholesterol |
0.2 mg |
Tween 80 |
5 mg |
Phenol red · Na |
21.24 mg |
Storage
- Aliquot unused portions into sterile tubes
- Store at 4 °C. Use within recommended shelf-life to maintain sterility and activity