Fastidious Anaerobe Agar (FAA, ATCC Medium: 2895) | For 7L

Fastidious Anaerobe Agar (FAA, ATCC Medium: 2895), for 7L

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Fastidious Anaerobe Agar (F.A.A.) is a Primary Isolation Medium for clinically significant anaerobes. It is optimised for the cultivation of anaerobic microorganisms and outperforms brain heart infusion agar, Brucella agar, and Wilkins-Chalgren agar, supporting the growth of over 80 fastidious strains with reduced hazing and improved visibility of beta-lactam endpoints, making it ideal for advanced clinical microbiology.

Functional Components:

- Peptones: Broad-spectrum growth stimulation

- Yeast extract: provides fuuband vitamins and microelements essential for growth

- Starch & Sodium Bicarbonate: reducing agents for detoxification

- Hemin: Promotes pigment production in P. melaninogenicus

- Whole-blood and additional yeast extract supplementation can enhance FAA's efficacy.

Selective Growth Promoters:

- Cysteine: Supports F. necrophorum, P. acnes, B. fragilis

- Arginine: Enhances Eubacterium spp.

- Soluble Pyrophosphate: Stimulates P. gingivalis, P. asaccharolyticus

- Pyruvate: Detoxifies and energises Veillonella spp.

- Vitamin K & Sodium Succinate: Essential cofactors for select strains

- Glucose (0.1%): Preserves colonial integrity by limiting acid/alcohol production

Applications

Fastidious Anaerobe Agar is suitable for culturing: Actinomyces, Anaerobes, Bacteroides, Brucella, Clostridium, Eubacterium, Fusobacterium, Haemophilus, Helicobacter, Neisseria, Peptostreptococcus, Porphyromonas, Propionibacterium, Streptococci

Formula

Tryptone ............................................................................... 23 g/L

Yeast Extract .......................................................................... 5 g/L

Sodium Chloride ..................................................................... 5 g/L

Soluble Starch ......................................................................... 1 g/L

Sodium Bicarbonate ............................................................ 0.4 g/L

Glucose .................................................................................... 1 g/L

Sodium Pyruvate ..................................................................... 1 g/L

L-Cysteine HCl .................................................................... 0.5 g/L

Sodium Pyrophosphate ..................................................... 0.25 g/L

L-Arginine ................................................................................ 1 g/L

Sodium Succinate ................................................................ 0.5 g/L

Hemin ................................................................................. 0.01 g/L

Vitamin K .......................................................................... 0.001 g/L

Agar ....................................................................................... 12 g/L

Final pH: 7.2 ± 0.2 at 25°C.

Components

[1]   Optional alternatives:

• Vitamin K₃ Stock
• Viscous liquid state of 98% Vitamin K₁ that has a longer shelf life at RT is also available, reagent pre-aliquoted into centrifuge tubes, each containing a sufficient amount to prepare a 20 mL stock solution at a final concentration of 0.2 mg/mL.

[2]   0.22µm filter sterilised.

Preparation

• Dissolve 36.75 g of Mixture A powder in 950 mL of Milli-Q water. 
• [Optional] If required, supplement with additional yeast extract
• Heat with frequent agitation and boil for one minute to completely dissolve the medium.
• Add 0.91 g of Mixture B and 1.00 g of Powder C to the broth. Mix well. Adjust the pH to 7.0–7.4.
• For a solid medium, add 12~15.0g Agar. For a semi-solid medium, add 0.5g Agar. Adjust the final volume to 1,000 mL with Milli-Q water.
• [Optional] Vacuum deoxygenate the broth prior to autoclaving, if desired.
• Autoclave at 121°C for 15 minutes.
• Cool the autoclaved FAA medium to 50–55 °C. Under an anaerobic atmosphere (AAE; e.g., N₂ 80% / H₂ 10% / CO₂ 10%, or N₂ 100%), aseptically add 5 ml of Vitamin K solution (Stock D) per 1,000 mL of medium (5µL/ml).
• [Optional] Blood Agar Preparation (if required).  For 5–10% blood agar, aseptically add the appropriate volume of sterile defibrinated blood to melted sterile agar medium.
• Spray anaerobe sample on top of the agar, incubate at 35 ± 2°C under anaerobic atmosphere(AAE) and examine for growth after 48 - 72 hours.
• Refrigerate(4 °C) the unused sterile medium; the shelf life is no more than 2~3 weeks.

Storage

• Mixture A and Mixture B, store at room temperature. Shelf life: 3 years.
• Powder C and Vitamin K Stock, store at 2~8 °C, protected from light.  Shelf life: 12 months.

When ordered with defibrinated blood, this item is shipped with 2.8 kg of dry ice for international deliveries and 1.3 kg of ice packs for domestic shipments.

For international customers, we recommend sourcing defibrinated sheep or horse blood locally whenever possible to minimise the risk of customs clearance delays, which are common when importing animal blood products.