Enriched Anaerobe medium (ATCC Medium 2210) | For 5L
- Product Code: GMNB-ATCC-EAM01
- Availability: Pre-Order
Tags: Enriched Anaerobe medium
Enriched Anaerobe medium (ATCC Medium 2210), for 5L
Enriched Anaerobe Medium (ATCC Medium 2210) serves as a cost-effective substitute for Chopped Meat Broth Medium (ATCC Medium 1490), where Chopped Meat Medium is the preferred formulation for culturing fastidious anaerobic bacterial species.
For bulk buy, please contact us for a quote via email support@gmexpression.com.
Applications
Enriched Anaerobe medium (ATCC Medium 2210) is suitable for:
- Pre-reduced, anaerobically sterilised (PRAS) media applications
- Cultivation, maintenance, and metabolic profiling of spore-forming and non-spore-forming obligate anaerobes
- Use as a holding medium for anaerobic cultures
- Promoting sporulation and toxin production in Clostridium species
- Gas-liquid chromatography (GLC) analysis of bacterial metabolic end products
|
Package contains |
5L pack |
|
Mixture A |
300.0 g |
|
Mixture B |
12.8 g |
|
Stock C (0.22µm filter sterilised) |
100 ml |
Components
|
Mixture A |
per 1000 ml |
|
Casitone (Casein peptone) |
30.0 g |
|
Defatted Beef Heart Extract |
20.0 g |
|
Yeast Extract (Premium Grade) |
5.0 g |
|
Dipotassium Phosphate (K₂HPO₄) |
5.0 g |
|
Resazurin |
1.0 mg |
|
Mixture B |
per 1000 ml |
|
Sodium Bicarbonate (NaHCO₃) |
2.0 g |
L-Cysteine·HCl |
0.5 g |
Hemin |
5.0 mg |
|
Stock C |
per 1000 ml |
|
Vitamin K₁ in Ethanol 0.05 mg/mL [1] |
20 ml |
[1] Optional alternatives: Viscous liquid state of 98% Vitamin K₁ that has a longer shelf life at RT is also available, reagent pre-aliquoted into centrifuge tubes, each containing a sufficient amount to prepare a 20 mL stock solution at a final concentration of 0.05 mg/mL.
Preparation
- Dissolve 60 g of Mixture A powder in 980 mL of Milli-Q water and mix well
- Boil the broth in a water bath or microwave for 5 minutes, and then cool the mixture under an anaerobic gas atmosphere (e.g., 80% N₂, 10% H₂, 10% CO₂, or 100% N₂).
- Add 2.5 g of Mixture B powder and 20 mL of Stock C
- Adjust the pH to 7.0.
- Dispense 7 mL into Hungate tubes or 1/5–1/4 volume of anaerobic culture bottles containing meat particles (1 part meat to 4–5 parts fluid) under the same anaerobic gas phase.
- Autoclave at 121 °C for 30 minutes.
For a solid medium, add 15.0g Agar. For a semi-solid medium, add 0.5g Agar. Dissolve together with Mixture A, to make 1000 mL of medium.
Storage
- Stock C: Store in a brown bottle at 2~8 °C, protected from light. Shelf life: 6 months.
