Differential Reinforced Clostridial Broth (DRCM)

Differential Reinforced Clostridial Broth (DRCM), 250g, 500g

Differential Reinforced Clostridial Broth is an enhanced version of the Reinforced Clostridial Medium, which was used to isolate and enumerate Clostridium species. 

The formulation of the medium includes the redox indicator resazurin for the verification of anaerobic conditions. A primary characteristic of this medium is its ability to provide a distinct visual checkpoint for the cultivation of Clostridia. During their metabolic activity, Clostridia reduce sulphite to sulphide, leading to the formation of an iron sulphide precipitate that turns the medium black. The selective isolation of these anaerobic, spore-forming microorganisms is further enhanced by the addition of polymyxin (70 IU/mL), which effectively suppresses the growth of contaminating non-spore-forming bacteria, such as non-clostridial organisms, that are also capable of sulphide production.

Broad Anaerobic Support

Promotes the growth of a wide range of anaerobes, including Clostridium spp.

Nutrient-Dense

Contains peptone, beef extract, and yeast extract to supply essential nitrogen, amino acids, vitamins, and carbon sources.

Fermentable Carbohydrate

Glucose (dextrose) supports bacterial metabolism.

Reducing Agent

Cysteine HCl lowers redox potential to maintain anaerobic conditions.

pH Stability

Sodium acetate acts as a buffering agent.

Osmotic Balance

Sodium chloride maintains appropriate osmotic pressure.

Semisolid Format

Low agar content facilitates anaerobic growth and motility studies.

Applications

Enumeration & Cultivation

For isolating and quantifying Clostridia from food, clinical, and environmental samples.

Food Microbiology

Detection of Clostridium spp. in dairy, canned, and processed foods.

Clinical Diagnostics

Isolation of Clostridia from clinical specimens.

Pharmacopoeial Testing

Compliant with USP and EP standards for non-sterile product testing.

Component, g/L

DRCM may be supplemented with polymyxin B for selective applications. Visit the product page of Polymyxin B Sulphate if needed.

* adjusted PH 6.9~7.3 as required 

Preparation

Suspend 23 g of the media powder in 1 L of distilled or Milli-Q water.

Heat till boiling with agitation until fully dissolved. Adjust PH to 6.9~7.3 as required.

Mix thoroughly and dispense 7 mL into Hungate tubes or 1/5–1/4 volume of anaerobic culture bottles under an anaerobic gas atmosphere (e.g., N₂-80%/H₂-10%/CO₂-10%, or N₂-100%).

Autoclave at 121 °C for 15 minutes.

For a solid medium, add 15.0g Agar. For a semi-solid medium, add 0.5g Agar to make 1000 mL of media.