• Mannitol Salt Agar

Mannitol Salt Agar

AUD 135.00    Excl.GST
  • Product Code: GMNB-MSA01
  • Availability: In Stock

Tags: Mannitol Salt Agar

Mannitol Salt Agar, 250g, 500g


Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. It is a selective medium prepared according to the recommendations of Chapman for the isolation of presumptive pathogenic staphylococci.


Applications

  • Mannitol Salt Agaris used for the selective isolation and differentiation of Staphylococcus aureus from clinical samples.
  • It is also used for the enumeration of staphylococci in food and dairy products.
  • This medium is also included in the Bacteriological Analytical Manual for cosmetics testing.
  • It is also used in the bacteriological examination of swimming pool water, spas and drinking water using membrane filtration.


Components

per 1000 ml

Casein Peptone

5.0 g

Peptic Digest of Animal Tissue

5.0 g

Beef Extract

1.0 g

Sodium Chloride

75.0 g

D-Mannitol

10.0 g

Phenol Red

0.025 g

Agar

15.0 g



Preparation

  1. Suspend 111.025 g of the media powder in 1000 ml of DI or MQ water.
  2. Heat till all media powder completely dissolved.
  3. Autoclave at 121 °C for 15~20 min.
  4. Cool the medium to approximately 50°C and pour into sterile Petri dishes.