MacConkey Agar | 250g
AUD 375.00
Excl.GST
- Product Code: GMNB-MCA01
- Availability: In Stock
Tags: Mannitol Salt Agar
MacConkey Agar, 250g
MacConkey agar (MAC) was the first solid differential media to be formulated which was developed at 20th century by Alfred Theodore MacConkey. MacConkey agar is a selective and differential media used for the isolation and differentiation of non-fastidious gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas.
Applications
- MacConkey agar is used for the isolation of gram-negative enteric bacteria.
- It is used in the differentiation of lactose fermenting from lactose non-fermenting gram-negative bacteria.
- It is used for the isolation of coliforms and intestinal pathogens in water, dairy products and biological specimens.
Components |
per 1000 ml |
Gelatin peptone |
17.0 g |
|
Proteose peptone |
3.0 g |
|
Lactose |
10.0 g |
|
Bile salts |
1.5 g |
|
Sodium chloride |
5.0 g |
Neutral Red |
0.03 g |
Crystal Violet |
0.001 g |
Agar |
13.5 g |
Preparation
- Suspend 50.03 g of the media powder in DI or MQ water. Make it 1000 ml.
- Heat till all media powder completely dissolved.
- Autoclave at 121 °C for 15 min.
- Cool the medium to approximately 50°C. Mix well and pour into sterile Petri dishes.
