BMMY Medium | 500mL
AUD 95.00
Excl.GST
- Product Code: GMNB-BMMY501
- Availability: In Stock
Tags: BMMY Medium
BMMY Medium | 1X stock | 500mL
International shipping will not include the Bottle of 10ml methanol due to Customs regulations. Please prepare the methanol separately.
Buffered Methanol- complex Medium
For Pichia pastoris culturing and recombination protein expression which is induced by methanol.
0.22μm filtration plus 5min 275/365nm UV treatment sterilized, Suggest storage in 4°C / 39.2 ℉ refrigerator.
Components
BMMY medium, per litre (final work solution)
1% Yeast extract
2% Peptone
0.1M Potassium phosphate (pH6.0)
1.34% YNB (Yeast Nitrogen Bases)
0.0004% Biotin
0.5% Methanol (separate stock)
Preparation
To prolong the shelf life of BMMY it is recommended to mix the methanol stock before each use.
After opening the bottle and pouring out the medium, mix 1.25 ml of 80X methanol stock per 100 ml of medium and use immediately, the remaining liquid should be sealed immediately and stored in a refrigerator at 4°C. The use of a sterile bench is recommended.
Notice: Due to the volatile nature of methanol, the shelf life of the 40% methanol stock (80X M 10ml) included in the package is up to 3 months and the accuracy of the concentration cannot be guaranteed after the expiry date. It is recommended that users prepare their own 50X or 80X methanol stock after the expiry date.
