Overview

Good's Buffer 1 M Solution Series - The Good's Buffer 1 M Solution Series has 12 ready-to-use Good's buffer stocks, together with HEPES, MOPS, and MES, covers the practical pH 5.5-11.0 biological-buffer range.


We also have

S30A Buffer · S30B Buffer · GMExpression Buffer catalogue 

Use and Applications

  • Protein purification, chromatography, and enzyme assay buffer formulation
  • Cell biology and CFPS buffer optimisation
  • Metal-sensitive workflows where phosphate and Tris are problematic
  • Custom pH screening across the Good's buffer family

→Buffer Indicator Navigator

Use the GMExpression Buffer Indicator Navigator to find suitable pH ranges that match this buffer system, or start from a specific buffer and search for suitable working pH ranges for the intended experiment.

Specifications

ItemSpecification
Included SKUsPIPES, BES, TES, ACES, TAPS, Tricine, Bicine, CHES, CAPS, EPPS/HEPPS, MOPSO, AMPSO
Companion existing/recommended SKUsHEPES, MOPS, MES
Custom pH serviceCustomer-specified pH within effective pH band
Bottle500 mL sterile bottle per buffer

Composition

ComponentAmount / concentrationFunction
PIPESpH 6.8; effective pH 6.1-7.5; CAS 5625-37-6Low-neutral pH Good's buffer
BESpH 7.1; effective pH 6.4-7.8; CAS 10191-18-1Neutral pH Good's buffer
TESpH 7.4; effective pH 6.8-8.2; CAS 7365-44-8Physiological pH Good's buffer
ACESpH 6.8; effective pH 6.1-7.5; CAS 7365-82-4Low-neutral Good's buffer
TAPSpH 8.4; effective pH 7.7-9.1; CAS 29915-38-6Mild alkaline Good's buffer
Tricine / BicinepH 8.0 / 8.3Protein electrophoresis and enzyme-assay buffers
CHES / CAPS / AMPSOpH 9.0-10.4 rangeAlkaline-range Good's buffers
EPPS / MOPSOpH 8.0 / 6.9Additional mid-range coverage

Instruction

1Choose the buffer whose effective pH range brackets the experimental pH.
2Dilute the 1 M stock to the desired final concentration, typically 10-100 mM.
3Confirm final pH after salts, detergents, cofactors, or temperature changes are introduced.
4For custom pH orders, specify buffer name, pH at measurement temperature, counter-ion preference, and required sterility level.

Note

  • Good's buffers are selected because they generally show low biological reactivity, useful pKa values, and lower metal interaction than phosphate or Tris in many workflows.

Cautions

Caution. No buffer family is universally inert. Validate compatibility with metal ions, UV exposure, redox chemistry, and assay readouts.
Caution. High-concentration Good's buffer stocks can be viscous and should be mixed thoroughly before aliquoting.

Storage and Expiry · Safety

  • Store at 2-8 deg C or room temperature as specified per SKU.
  • Unopened shelf life target: 24 months from manufacture after QC validation.
  • Transport. Ships at ambient temperature; no cold chain required. Protect from freezing and from temperatures above 40 deg C.
  • Handling and PPE. Wear a laboratory coat, nitrile gloves, and safety glasses for routine handling. The formulated buffer presents no significant hazard under normal use; avoid ingestion and eye contact. Consult the Safety Data Sheet before use.

References

  1. Good et al. (1966), Biochemistry 5:467-477.

Frequently Asked Questions

Q1. Why buy the full set?
The set supports rapid pH optimisation without in-house preparation of many individual buffer stocks.
Q2. Can pH points differ from the default table?
Yes. Customer-specified pH points within each buffer's effective range are a natural extension of the series.