DNA Booster 50X Stock, without LB | 50ml

DNA Booster 50X Stock (version 1.2) without LB | 50ml

Prepare up to 2.5 litres of liquid medium

0.22μm filtration plus 5min of 275/365nm UV treatment sterilised.

This is a unique Media Enhancement Stock developed by GMExpression®. It has been designed to increase the yield of the bacterial plasmid for the most commonly used liquid media.

It gives an optimum yield of transformed bacteria in culture, resulting in a much higher plasmid yield per volume than traditional LB media.

Recommended to use

• Suitable for preparing plasmids of bacteria such as E.coli in the laboratory.
• Suitable for the recovery of electroporation-transformed bacteria.
• Suitable for the preparation of competent cells.
• Reduce the cultivation time of the host bacteria, ideal for the rapid preparation of DNA.
• The DNA Booster can also be used to prepare auto-induction media to enhance E. coli protein expression efficiency. We recommend starting with a relatively low concentration, such as adding 1 volume of 50X stock to 100~200 volumes of media.

Composition

Booster formula

High-density cell growth maintenance component (GMExpression®)

Plasmid/gDNA Anabolic Enhancement Component (GMExpression®)

Preparation and storage

• For high-copy number plasmid preparation, use the dark colour stock, and for low-copy number plasmid uses the light colour one.
• Unopened stock can be stored at RT, but refrigeration is recommended. After opening, the remaining liquid should be sealed immediately and stored in the 4°C (39.2°F) refrigerator.
• Operation on a sterile bench is recommended.
• Users need to prepare either LB, SOB or 2YT sterilised liquid media in advance.
  
• Dilute 1 volume of 50X stock to 49 volumes of liquid media (LB, SOB or 2YT; e.g. add 10 ml of 50X stock to 490 ml of LB medium to make 500 ml DNA Booster medium), shake well and use in the same way as a normal LB medium.
• For E.coli (like TOP10, DH5a strain) incubation, incubate for 2-3 hours to obtain highly active bacteria (in the log phase of growth), for 4 hours of rapid incubation (plateau growth) or for overnight incubation.

Caution

• This stock solution or mixed medium should not be autoclaved. It should only be sterilised by 0.22µm filtration.
• The formulas for our proprietary products are constantly updated as our back-end research continues, with newer versions of the products being released to the shelves when the results of subsequent development show improved performance.
• The data shown in the figure is the actual performance of the Version 1.0 product (as of February 2023). The Version 1.2 product has a 10~20% better performance compared to Version 1.0