DNA Booster, plasmid specific medium | 500ml

DNA Booster Plasmid Specific medium, LB Media Based, v1.2 | 500ml

0.22μm filtration plus 5min 275/365nm UV treatment sterilised.

This is a unique Media Enhancement Stock developed by GMExpression®. It has been designed to increase the yield of the bacterial plasmid for the most commonly used liquid media.

It gives an optimum yield of transformed bacteria in culture, resulting in a much higher plasmid yield per volume than traditional LB media.

Recommended to use

• Suitable for preparing plasmids of bacteria such as E.coli in the laboratory.
• Suitable for the recovery of electroporation-transformed bacteria.
• Suitable for the preparation of competent cells.
• Reduce the cultivation time of the host bacteria, ideal for the rapid preparation of DNA.

Composition

Booster formula

High-density cell growth maintenance component (GMExpression®)

Plasmid/gDNA anabolic enhancement component (GMExpression®)

Preparation and storage

• Unopened media can be stored at RT, but refrigeration is recommended. After opening, the remaining liquid should be sealed immediately and stored in the 4°C (39.2°F) refrigerator.
• Pay attention to the different media for different uses. The dark colour media is specific for high-copy number plasmid preparation, and the light colour media is specific for low-copy number plasmid preparation.
• Operation on a sterile bench is recommended.
• For E.coli (e.g. TOP10, DH5a strain) incubation, incubate for 2~3 hours to obtain highly active bacteria (in the log phase of growth), for 4 hours of rapid incubation (plateau growth) or overnight incubation.

Caution

• This media has been sterilised by 0.22µm filtration, and can be used directly.
• The formulas for our proprietary products are constantly updated as our back-end research continues, with newer versions of the products being released to the shelves when the results of subsequent development show improved performance.
• The data shown in the figure is the actual performance of the Version 1.0 product (as of February 2023). The Version 1.2 product has a 10~20% better performance compared to Version 1.0