Todd-Hewitt Broth, with 2% w/v Yeast Extract
- Product Code: GMNB-THY01
- Availability: In Stock
Todd-Hewitt Broth, with 2% w/v Yeast Extract, 250g
This enriched medium is formulated for the cultivation of streptococci and other fastidious microorganisms, with particular utility in serological testing and antigen production. The addition of yeast extract provides essential nutrients, including B vitamins, to support vigorous growth.
Applications
Todd-Hewitt Broth, with 2% w/v Yeast Extract, is suitable for:
- Cultivation: Promotes growth of Streptococcus spp., including groups A and B, as well as other nutritionally demanding organisms.
- Serological testing: Recommended for preparing streptococci for group-specific serological assays.
- Antigen production: Supports the generation of streptococcal antigens for diagnostic and research purposes.
- Enrichment: Effective for detecting Streptococcus agalactiae (group B) in specialised applications such as immunofluorescence.
Components |
per 1000 ml |
|
Peptone |
20.0 g |
|
Yeast Extract (Premium Grade) |
20.0 g |
|
Beef heart infusion |
3.1 g |
|
Sodium carbonate |
2.5 g |
|
Sodium chloride |
2.0 g |
|
Glucose |
2.0 g |
|
Sodium hydrogen phosphate |
0.4 g |
Preparation
- Dissolve 50g of the media powder in 1000 ml of DI or MQ water.
- [Optional] For making Todd-Hewitt Broth with Tween, add 1.0 ml of Tween 80..
- Adjust PH to 7.6~8.0.
- The broth can be prepared in aerobic or anaerobic conditions according to the different culture purposes. [1]
- Autoclave at 121 °C for 15 min.
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[1] Streptococcus hemolyticus is a facultative anaerobe. It is capable of aerobic respiration in the presence of oxygen, but can also generate energy through fermentation under oxygen-limited conditions. These organisms grow well in aerobic environments; however, under anaerobic conditions, their hemolytic activity may become more.
