Overview

Tyrode's Solution, Modified, 10x Stock - Tyrode's Solution is a classical physiological salt solution for cardiac muscle, smooth muscle, and platelet biology. The GMExpression candidate is a 10x modified carbonate-free stock designed for shelf stability and fast point-of-use preparation.

The product sits naturally in the Physiological Perfusate Family beside KHB and aCSF, giving pharma and academic physiology laboratories a consistent domestic supplier for three common perfusates.

We also have

Artificial Cerebrospinal Fluid · Krebs-Henseleit Buffer · GMExpression Buffer catalogue 

Use and Applications

  • Cardiac safety pharmacology and isolated tissue bath experiments
  • Smooth muscle physiology studies
  • Platelet aggregation and coagulation-adjacent workflows
  • General physiological salt-solution preparation where Tyrode's composition is specified

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Specifications

ItemSpecification
Bottle500 mL sterile 10x stock
Buffering designHEPES included; bicarbonate companion optional where protocol requires carbogen
Sterility0.1 μm (mycoplasma-reduction grade) sterile-filtered; released mycoplasma-test-negative (PCR)
BundlePhysiological Perfusate Family with KHB and aCSF

Composition

ComponentAmount / concentrationFunction
NaCl137 mM at 1x targetMajor extracellular ion
KCl2.7 mM at 1x targetPotassium component
NaH2PO40.42 mM at 1x targetPhosphate component
MgCl21.05 mM at 1x targetMagnesium component
CaCl21.8 mM at 1x targetCalcium component
D-glucose5.5 mM at 1x targetMetabolic substrate
HEPES10 mM at 1x targetCO2-independent buffering

Instruction

1Dilute 1 volume of 10x stock with 9 volumes of sterile ultrapure water (0.1μm filterated MQ water).
2If the bicarbonate/carbogen protocol is required, add NaHCO3 after dilution and equilibrate with carbogen.
3Warm to the protocol-specified temperature before use in organ bath or platelet assays.
4Use diluted 1x solution within 24 hours.

Note

  • Tyrode's formulations vary by discipline. This page describes the modified shelf-stable stock; custom Ca-free, HEPES-only, or bicarbonate-forward variants are feasible. Quotation separately via support@gmexpression.com

Cautions

Caution. Do not store diluted bicarbonate-containing Tyrode's for long periods.
Caution. Check calcium and magnesium requirements carefully for platelet and smooth-muscle protocols.

Sterile Filtration - Mycoplasma-Reduction Grade

Because this solution is used with living mammalian cells and tissue, it is sterilised through a 0.1 μm membrane rather than the conventional 0.22 μm sterilising grade. Mycoplasma are deformable enough to pass through 0.22 μm pores; where a 0.1 μm filteration gives a substantially lower mycoplasma margin, and each lot is released mycoplasma-test-negative by PCR.

If your experimental subjects are virus-sensitive cells, organoid or tissue samples, or if you wish to ensure that the experimental results are free from viral interference, we also provide Nanofiltration 1X stock (0.015 μm). Quotation separately via support@gmexpression.com.

Storage and Expiry · Safety

  • Store 10x stock at 2-8 deg C.
  • Unopened shelf life: 24 months from manufacture after QC validation.
  • Transport. Ships at ambient temperature in insulated packaging; brief transit excursions are tolerated. Refrigerate at 2-8 deg C on receipt and protect from freezing.
  • Handling and PPE. Wear a laboratory coat, nitrile gloves, and safety glasses for routine handling. The salt stock presents no significant chemical hazard; maintain aseptic technique. Consult the Safety Data Sheet before use.

References

  1. Tyrode (1910), Archives Internationales de Pharmacodynamie et de Therapie 20:205-223.
  2. Safety Data Sheet: SDS draft to be finalised before commercial release.

Frequently Asked Questions

Q1. Is this the same as KHB?
No. Both are physiological salt solutions, but Tyrode's has a different ion profile and is used for different tissue systems.
Q2. Can HEPES-free Tyrode's be supplied?
Yes. A bicarbonate/carbogen-only variant is feasible as a custom formulation. Quotation separately via support@gmexpression.com