Yeast Extract | Cold Extract Lysate | 2kg Industrial Package
- Product Code: IDA-YEIAA
- Availability: In Stock
Tags: Cold lysate Extract
Yeast cold lysate extract
Basic medium ingredient for large-scale (Industrial) protein expression use | 2 kg package
Suitable for induced protein expression and bacteria-culture that requires strict restrictions on auto-induction.
This product has a relatively short shelf life. Purchase of more than 2kg requires a pre-order. The lead time is about 6~8 weeks.
This yeast cold lysate extract is derived from brewer’s yeast (Saccharomyces cerevisiae) through a high-pressure injection lysis process, followed by protease hydrolysis, concentration, and vacuum powdering. The extract undergoes complete enzymatic digestion, yielding premium protein-nitrogen content primarily in the form of free amino acids and balanced micronutrients—exceeding 5% protein nitrogen (compared to ~4% in conventional yeast extracts).
The cold extraction preserves active intracellular components, including reduced glutathione (GSH), while minimising oxidative metabolites. As a result, it offers a highly bioavailable, low-reactivity nutrient profile—ideal for sensitive microbial and eukaryotic cell cultures.
Notably, the extract contains significantly fewer oligo- and monosaccharides—typically derived from cell wall polysaccharide hydrolysis—that can act as expression inducers (e.g., mannose, galactose). This helps reduce unwanted metabolic activation and supports controlled culturing environments.
Properties
Total nitrogen (N) basis ≥ 10.0%
Amino-nitrogen basis ≥ 5.0%
Residue on ignition ≤15.0%
Loss on drying ≤6.0%
Sodium chloride ≤2.0%
pH (2% in H2O) 5.3~7.2
Heavy metal content
Lead <1.9mg/kg
Arsenic <2mg/kg
Microbiological indicators
Total number of bacterial colonies <10kCFU/g
Escherichia coli 0.3MPN/g
Salmonella enterica Not detected
Staphylococcus aureus Not detected
Form
Powder, yellow to light yellow
Solubility
H2O: 2%, clear, yellow
FAQ
Compare Different Grades of Yeast Extract products:
| Yeast Powder – Industrial Grade | Yeast Extract – Economic Grade (Industrial Grade) | Yeast Extract – Standard Grade | Yeast Extract – Premium Grade | |
| Source | Lager beer yeast (fewer wild bacteria) | High-protein bread yeast cultured on starch solid media | High-protein bread yeast cultured on starch solid media | High-protein bread yeast cultured on starch solid media |
| Characteristics | Non-autolysed; retains essential vitamins and trace elements | Reduced plant-derived pigment (tannic acid) | Elevated peptide content | Minimal heat treatment preserves biotin, riboflavin, and other temperature-sensitive molecules |
| Total nitrogen | ≥ 7.5 % | ≥ 9.0 % | ≥ 10.0 % | ≥ 10.0 % |
| Amino-nitrogen | ≥ 2.5 % | ≥ 2.5 % | ≥ 4.0 % | ≥ 5.0 % |
| Solubility | < 2 % | < 2 % | 2 % | 10 % |
| Applications | Feeding fruit flies or laboratory animals | Affordable protein-scale expression and general microbial culturing | Routine lab assays and bacterial culturing requiring high yield | Clinical diagnostics, pharmaceutical research, and high-precision experiments |
| Pricing | AUD 450.00 - 10 kg | AUD 575.00 - 10 kg | AUD 780.00 - 10 kg | AUD 995.00 - 10 kg |
| Availability | Pre-order; 6–8 weeks lead time | Pre-order; 6–8 weeks lead time | Pre-order; 6–8 weeks lead time | Pre-order; 6–8 weeks lead time |
Yeast Cold Extract Lysate – High-End Formulation
- Characteristics: Retains active intracellular enzymes and maintains the medium’s redox micro-environment
- Applications: Demanding protein/molecule expression workflows for fastidious organisms
