PDA medium, Potato Dextrose Agar | 250g
- Product Code: GMNB-PDA01
- Availability: In Stock
Tags: PDA medium
PDA medium, Potato Dextrose Agar| 250g
Potato Dextrose Agar is a medium used for the cultivation of bacteria, fungi, yeasts and moulds.
The relatively low pH of the PDA medium allows the selective cultivation of acid-tolerant yeasts, fungi, bacteria and moulds, making it suitable for the study of ascospore dispersal.
Potato extract acts as a source of carbon, nitrogen, minerals, vitamins and other essential growth nutrients, while dextrose acts as a source of carbohydrate.
PDA medium is suitable for use in:
- Isolation and enumeration of yeasts and moulds from food and dairy products
- Stimulation of sporulation
- Maintaining stock cultures of fungi
- Differentiation of various dermatophytes based on pigment production
Per 1000mL of PDA medium contains
Potato Extract (premium grade) 6g
Glucose 20g
Agar * 15g
Chloramphenicol 0.1g（Optional）
* Tissue Culture Grade | Gel strength (15g/L, 20°C) ≥1400g/cm2
Microbial sensitivity test
Prepare the Potato dextrose agar (containing chloramphenicol) according to the standard formula, inoculate the following quality control strains, and aerobic cultivation at 28±1 degrees celsius for 48-96 hours.
|
Quality control strain |
strain number |
Inoculum volume (CFU) |
counting medium |
method |
Quality control results
|
Appearance characteristics |
|
Aspergillus niger |
ATCC16404 |
20-200 |
PDA |
Quantitative |
PR > 0.7 |
There are black spores and white hyphae |
|
Saccharomyces cerevisiae |
ATCC9763 |
20-200 |
PDA |
Quantitative |
PR > 0.7 |
cream colored colonies |
|
Candida albicans |
ATCC10231 |
20-200 |
PDA |
Quantitative |
PR > 0.7 |
cream colored colonies |
Preparation
Weigh 41 g of PDA medium, dissolve in distilled or MQ water and make the final volume to 1L. Autoclave at 121°C for 15 minutes.
