• PDA medium, Potato Dextrose Agar | 250g

PDA medium, Potato Dextrose Agar | 250g

AUD 135.00    Excl.GST
  • Product Code: GMNB-PDA01
  • Availability: In Stock

Tags: PDA medium

PDA medium, Potato Dextrose Agar| 250g

Potato Dextrose Agar is a medium used for the cultivation of bacteria, fungi, yeasts and moulds. 

The relatively low pH of the PDA medium allows the selective cultivation of acid-tolerant yeasts, fungi, bacteria and moulds, making it suitable for the study of ascospore dispersal. 

Potato extract acts as a source of carbon, nitrogen, minerals, vitamins and other essential growth nutrients, while dextrose acts as a source of carbohydrate.


PDA medium is suitable for use in:

  • Isolation and enumeration of yeasts and moulds from food and dairy products
  • Stimulation of sporulation
  • Maintaining stock cultures of fungi
  • Differentiation of various dermatophytes based on pigment production


Per 1000mL of PDA medium contains

Potato Extract (premium grade)      6g

Glucose                                             20g

Agar *                                                15g

Chloramphenicol                              0.1g(Optional)


* Tissue Culture Grade | Gel strength (15g/L, 20°C) ≥1400g/cm2


Microbial sensitivity test

Prepare the Potato dextrose agar (containing chloramphenicol)  according to the standard formula, inoculate the following quality control strains, and aerobic cultivation at 28±1 degrees celsius for 48-96 hours.

Quality control strain

strain number

Inoculum volume (CFU) 

counting medium  

method

Quality control results           

Appearance characteristics

Aspergillus niger

ATCC16404

20-200

PDA

Quantitative

PR > 0.7

There are black spores and white hyphae

Saccharomyces cerevisiae

ATCC9763

20-200

PDA

Quantitative

PR > 0.7

cream colored colonies

Candida albicans    

ATCC10231

20-200

PDA

Quantitative

PR > 0.7

cream colored colonies

  


Preparation

Weigh 41 g of PDA medium, dissolve in distilled or MQ water and make the final volume to 1L.  Autoclave at 121°C for 15 minutes.