Streptomycin sulfate | 100g
- Product Code: CH-PP-SS01
- Availability: 4-6 weeks
Tags: Streptomycin sulfate
Streptomycin sulfate | 100g
CAS Number: 3810-74-0
Linear Formula: C21H39N7O12 · 1.5 H2SO4
Molecular Weight: 728.69
Storage: 2~8℃
Grade: High purity grade
Potency: 750-850mcg/mg
Moisture: ≤2.0%
Properties:
white to off-white powder.
Uses
- Cell culture
Streptomycin is used to study mechanisms of streptomycin resistance and is often used together with penicillin and other agents to inhibit bacterial contamination in cell culture applications. It is recommended for use in molecular biology applications at 25-50μg/mL, in cell culture applications at 100 mg/L and in embryo culture at 50 mg/L.
- Mode of Action:
Streptomycin acts by inhibiting prokaryote protein synthesis by binding to the S12 protein of the 30S ribosomal subunit, thus preventing the transition from the imitation complex to chain-elongating ribosome. This causes miscoding or inhibits initiation.
- Protein purification
When purifying protein from a biological extract, streptomycin sulfate can be added to remove nucleic acids and ribonuclear proteins. it can bind to ribosomes and precipitate out of solution, it serves as a method for removing rRNA, mRNA, and even DNA if the extract is from a prokaryote.
Cautions
Stock requires storage at -20°C after the antibiotic has been configured into the solution.
This product is restricted to scientific research only and should not be used for clinical therapy or other purposes.