SOC Medium, SOC media, 250g
AUD 99.00
Excl.GST
- Product Code: GMNB-SOC117
- Availability: In Stock
SOC Medium
Super Optimal broth with Catabolite repression, for transforming bacteria use.
250 g. Fine powder, up to make 7.9L liquid media.
Components
0.5% Yeast extract (premium)
2% Tryptone
10 mM NaCl
2.5 mM KCl
10 mM MgCl2
10 mM MgSO4
20 mM Glucose
Preparation
Suspend 31.4 g in 950 ml distilled water, mix well and then make up to 1000ml.
Sterilise by autoclaving at 115℃ for 30 minutes×. Aliquot as needed.
×Monosaccharides are susceptible to Maillard reaction with amino acids in a high-temperature environment, therefore sterilization at 121 degrees for 15 minutes is not recommended. Alternatively, 0.22 µm filtration can be used as a substitute for autoclaving if conditions permit.
