Mycoplasma Eliminating Cocktail | 10ml
AUD 275.00
Excl.GST
- Product Code: ATB-M-MEC01
- Availability: In Stock
Mycoplasma Elimination Cocktail | 10ml (2.5 µmol)
- Rapid elimination of multiple mycoplasma species
- Prevent (re)contamination of cell cultures
- Compatible with most mammalian cell lines
- Very low cytotoxicity and preserves cell viability
- Very low resistance escape rate of mycoplasma
Active ingredient: 3 quinoline derivatives with a total of 250 pmol in 1 ml of water.
4-oxo-1,4-dihydro-3-quinolinecarboxylic acid CAS Number: 34785-11-0 42.5µg/ml (225 pmol/ml)
Ofloxacin CAS Number: 82419-36-1 4.5µg/ml (12.5 pmol/ml)
Levofloxacin CAS Number: 138199-71-0 4.5µg/ml (12.5 pmol/ml)
Mycoplasma Elimination Cocktail (MEC) is an effective reagent for the elimination of various types of mycoplasma from contaminated cell cultures.
MEC can also be used to prevent contamination of cured cultures by mycoplasma.
The product is a combination of 3 different types of 4-oxo-quinoline-3-carboxylic acid derivatives that inhibit mycoplasma replication by inhibiting the topoisomerase enzyme of mycoplasma DNA, thereby eliminating mycoplasma infection.
MEC is very robust, easy to use and fast-acting, for most cell cultures contaminated with mycoplasma, simply add the appropriate amount of MEC to cell cultures and incubate for one week.
This product should be stored undiluted. Store at room temperature, protected from light.
Instructions
1. Add MEC at a final concentration of 2.5 pmol/ml to cell cultures contaminated with mycoplasma and incubate for one week.
2. For media replacement or culture transfer (passage), use a medium containing MEC at 2.5 pmol/ml.
3. Transfer the cell cultures several times without MEC and confirm that there has been no regrowth of the contaminating mycoplasma.
MEC at a concentration of 1.25 pmol/ml may be added to the media to prevent mycoplasma contamination of cell cultures that are exposed to serum or trypsin.
Note:
The typical treatment is recommended to use MEC at a concentration of 2.5 pmol/ml, this concentration may be increased to 7.5 pmol/ml only if the recommended concentration is not effective in eliminating mycoplasma.
Although MEC is very low cytotoxic when used at the recommended concentration, for the specific function of cultured cells, confirmation of the maintenance of the desired cellular characteristics after MEC treatment is advised.
This product is restricted to scientific research only and should not be used for clinical therapy or other purposes.
