Guanidine thiocyanate / Guanidine isothiocyanate
CAS No.:593-84-0
Molecular Formula: CH5N3.CHNS
Molecular weight:118.16
EINECS No.:209-812-1
Purity: 99%, 99.5%
Appearance: white crystalline powder, soluble in water
Application: Biochemical research. Molecular biology protein denaturation. RNA preparation
Store: RT
Guanidine isothiocyanate is a powerful protein denaturant with no RNAase or DNAase activity. In nucleic acid extraction, guanidinium isothiocyanate can rapidly break cells or viruses to release nucleic acid, inhibit the nuclease released from the cells to ensure the integrity of the primary structure of nucleic acid, commonly used in RNA extraction. Due to autologous degradation and enzyme-mediated degradation, RNA is a biomolecule that is difficult to stabilise and preserve, and as the main component of nucleic acid lysate, guanidine isothiocyanate is a powerful protein denaturant, which is capable of destroying the molecular structure of the enzyme RNA, and then inactivate the nuclease that may be present in the sample, thus stabilising the RNA sample.
