Brucella Agar | with Hemin & VK1 / Defibrinated Blood | for 6L

Brucella Agar (Remel Brucella Agar) | with Hemin & VK1 or Sheep Blood | for 6L

-  Brucella Agar with Hemin and Vitamin K1 

-  Brucella Agar with 5% Sheep/Horse Defibrinated Blood

Use

Brucella Agar is a standard formulation used for the isolation of fastidious bacteria, Streptococci, Pneumococci, Listeria, Brucella, Neisseria meningitidis and Haemophilus influenzae. It is also recommended as a primary nonselective isolation medium for Helicobacter pylori.

-  For strict anaerobes, especially Bacteroides, Prevotella and Porphyromonas when incubated anaerobically, including isolation of strict anaerobes from clinical specimens and anaerobic susceptibility testing using the E test method. Brucella blood agar with haemin and vitamin K1 is preferred.

-  For isolation and growth of fastidious and non-fastidious bacterial species by detection of haemolytic reactions, including isolation of Brucella from clinical specimens. Brucella agar with 5% Sheep/Horse Defibrinated Blood is preferred.

Cautions

·  Laboratory procedures involving Brucella require special equipment and techniques to minimise biohazards.

·  Biosafety level 3 is required for handling samples and cultures.

·  This medium should not be used as a universal primary isolation medium.

Per 1000mL of Brucella Agar contains

*  Adjusted and/or supplemented as required to meet performance criteria.

*  Blood products have a maximum shelf life of 35 days， and the Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Please consider the requirements before purchasing.

**  Haemolytic reactions are unavailable if using Blood Alternative to prepare the media.

Preparation

-       Dissolve 43.1 g of mixture A in 930 ml of MQ water in a closed container， Adjust the pH to PH6.8~7.2 using 1M NaOH or 1M HCl. 

-       Autoclave at 121°C for 15 minutes (Nitrogen blanket autoclave recommended ^[1][2]; if the condition does not allow, see [3]).

-       Add 10 ml of stock B, and 50 ml of optional stock C or Stock D if required, to the 930 ml solution and make it 1000 ml at 50~55°C. Shake well and pour the agar plate aseptically while the liquid is still hot.

[1]     Inert gas blanketing, also known as nitrogen gas protection, is a safe and reliable method of preserving volatile or oxidatively sensitive substances.

[2]     A simple method of inert gas blanketing in the laboratory:

-   GL45-cap reagent bottles filled with the liquid to be treated are placed in an airtight PP autoclave bag - provided in the pack (the bag is best placed on top of a stainless steel tray or in a steel mesh basket)

-   Squeeze as much air out of the bag as possible, then introduce nitrogen into the bag using a flexible tube and seal the bag (by tying a knot at the neck of the bag or using a heat-resistant rubber band - provided in the pack) when it is inflated to about half its maximum volume.

-   Place the tray in a microwave oven to boil the liquid or in an autoclave chamber to sterilise.

-   Be aware of the risk of asphyxiation from high concentrations of nitrogen and ensure that the operation is carried out in a ventilated room.

[3]     If inert gas protection is not intended,

-   GL45-cap reagent bottles filled with the liquid to be treated are placed in an airtight PP autoclave bag - provided in the pack (the bag is best placed on top of a stainless steel tray or in a steel mesh basket).

-   Squeeze as much air out of the bag as possible, then seal the bag (by tying a knot at the neck of the bag or using a heat-resistant rubber band - provided in the pack) immediately.

-   Place the tray in a microwave oven to boil the liquid or in an autoclave chamber to sterilise.

Note:

It is strongly recommended to operate within a clean bench when preparing the solution & agar plate.

When ordering with the blood stock, this item will be packaged with 2.8kg of dry ice as standard for international shipping. For domestic shipping, it is packaged with a 1.3kg ice bag.

International buyers are suggested to purchase defibrinated sheep blood or horse blood locally as far as possible, unless it is difficult to do so, to avoid risks in customs clearance at the point of entry, which is common for importing animal blood products.