Anaerobic Preparation Kit

Anaerobic Preparation Kit Lite 

Used for vacuum-style media preparation without nitrogen blanketing,  This kit has been included in the YCFA Full Recipe (GMNB-YCFA03) by default. which contains:

1X 25 cm PTFE tube[1]

1X φ 25 mm 0.22 µm disposable filter

2X Luer male connector[1]

1x Three-way Luer connector[1]

2X Check valve (prevents backflow)[1]

1X 100 ml syringe for fast vacuum[1]

3X 23G syringe needle

1X GL45 PBT cap[1][2] with 1x Butyl Rubber stopper[2][3]

Anaerobic Preparation Kit Plus 

which contains:

Module A for vacuum-style preparation without nitrogen blanketing

1X 25 cm PTFE tube[1]

1X φ 25 mm 0.22 µm disposable filter

3X Luer male connector[1]

2X Three-way Luer connector[1]

2X Check valve (prevents backflow)[1]

1X 100ml syringe for vacuum[1]

1X GL45 PBT cap[1][2] with 2X Butyl Rubber stopper[2][3]

Module B for preparing nitrogen from air (deoxygen)

Nitrogen (deoxygenated air) can be further introduced into the GL45 bottle through Module A after vacuuming to best protect media from oxygen.

1x Autoclave bag airtight seal stick[1]

2x PTFE air valve can attach to autoclave bag[1]

10X PP pressed self-sealing autoclave bag[1][2][4]

10X 500cc oxygen absorber[1][2]

5X Oxygen indicator paper[1][2]

1X 25 cm PTFE tube[1]

[1]. Reusable

[2]. Can be purchased separately

[3]. Max 7 times of needle puncture

[4]. Not the pp autoclaving bags provided along with the YCFA media

[5] PBT cap with a Butyl Rubber stopper, which can be purchased separately (price: PBT cap AUD 13 ea and Butyl Rubber stopper AUD 9 ea).