Anaerobic Preparation Kit Lite
Used for vacuum-style media preparation without nitrogen blanketing, This kit has been included in the YCFA Full Recipe (GMNB-YCFA03) by default. which contains:
1X 25 cm PTFE tube[1]
1X φ 25 mm 0.22 µm disposable filter
2X Luer male connector[1]
1x Three-way Luer connector[1]
2X Check valve (prevents backflow)[1]
1X 100 ml syringe for fast vacuum[1]
3X 23G syringe needle
1X GL45 PBT cap[1][2] with 1x Butyl Rubber stopper[2][3]
Anaerobic Preparation Kit Plus
which contains:
Module A for vacuum-style preparation without nitrogen blanketing
1X 25 cm PTFE tube[1]
1X φ 25 mm 0.22 µm disposable filter
3X Luer male connector[1]
2X Three-way Luer connector[1]
2X Check valve (prevents backflow)[1]
1X 100ml syringe for vacuum[1]
1X GL45 PBT cap[1][2] with 2X Butyl Rubber stopper[2][3]
Module B for preparing nitrogen from air (deoxygen)
Nitrogen (deoxygenated air) can be further introduced into the GL45 bottle through Module A after vacuuming to best protect media from oxygen.
1x Autoclave bag airtight seal stick[1]
2x PTFE air valve can attach to autoclave bag[1]
10X PP pressed self-sealing autoclave bag[1][2][4]
10X 500cc oxygen absorber[1][2]
5X Oxygen indicator paper[1][2]
1X 25 cm PTFE tube[1]
[1]. Reusable
[2]. Can be purchased separately
[3]. Max 7 times of needle puncture
[4]. Not the pp autoclaving bags provided along with the YCFA media
[5] PBT cap with a Butyl Rubber stopper, which can be purchased separately (price: PBT cap AUD 13 ea and Butyl Rubber stopper AUD 9 ea).